differentiation and apoptosis of u937 leukemia cells by an active compound from dendrostellera lessertii
نویسندگان
چکیده
background: regarding the strong differentiation and anti-leukemic activity of dendrostellera lessertii extract, an active agent with similar capabilities was isolated from the crude extract of the plant leaves. the aim of this study was to determine whether the anti-proliferative effect observed for this compound, is differentiation-dependent among the drug-treated cells, using u937 cells. methods: monocyte differentiation was evaluated by wright-giemsa staining, latex particle assay and flow cytometry. induction of apoptosis was analyzed by annexin-pi double staining. results: the new compound, at 0.5-2.5 µg/ml inhibited proliferation of u937 cells by more than 70% and their viabilities were decreased by 47 ± 2.1% after 72 h of treatments. in addition, we found that the effect of the new compound on u937 cells was associated with differentiation toward monocyte/macrophage lineage based on nitroblue tetrazolium reduction assay, morphology change, phagocytic activity and expression of cell surface markers (cd14 and cd11b) as analyzed by flow cytometry. moreover, our results indicated that the treatment of u937 cells with the new compound for 3 to 4 days induced apoptosis as assayed qualitatively by acridine orange/ethidium bromide and annexin-v/pi double staining technique using flow cytometry. conclusion: based on these observations, it is concluded that the anti-proliferative function of the new compound is exerted through differentiation-dependent apoptosis among the treated cells, similar to the function of 3-hydrogenkwadaphnin previously characterized from d. lessertii crude extract.
منابع مشابه
Differentiation and Apoptosis of U937 Leukemia Cells by an Active Compound from Dendrostellera lessertii
Background: Regarding the strong differentiation and anti-leukemic activity of Dendrostellera lessertii extract, an active agent with similar capabilities was isolated from the crude extract of the plant leaves. The aim of this study was to determine whether the anti-proliferative effect observed for this compound, is differentiation-dependent among the drug-treated cells, using U937 cells. Me...
متن کاملDifferentiation and apoptosis of U-937 leukemia cells by an active compound from Dendrostellera lessertii.
BACKGROUND Regarding the strong differentiation and anti-leukemic activity of Dendrostellera lessertii extract, an active agent with similar capabilities was isolated from the crude extract of the plant leaves. The aim of this study was to determine whether the anti-proliferative effect observed for this compound, is differentiation-dependent among the drug-treated cells, using U937 cells. ME...
متن کامل3-Hydrogenkwadaphnin from Dendrostellera lessertii induces differentiation and apoptosis in HL-60 cells.
3-Hydrogenkwadaphnin (3-HK) is a new diterpene ester, recently isolated from the leaves of Dendrostellera lessertii with potent anti-tumoral and anti-metastatic activities. Herein, we report that 3-HK induces differentiation and apoptosis in HL-60 cells. The drug at 2.5 - 40 nM inhibited proliferation of HL-60 cells after 24 - 96 h of treatment. Cell viability was also decreased by 57 % after 9...
متن کاملAtromentin-induced apoptosis in human leukemia U937 cells.
In the course of screening for apoptotic substances that induce apoptosis in human leukemia U937 cells, a fungal strain, F000487, which exhibits potent inducible activity, was selected. The active compound was purified from an ethyl acetate extract of the microorganism by Sep-pak C18 column chromatography and HPLC, and was identified as atromentin by spectroscopic methods. This compound induced...
متن کاملG1 Phase Arrest and Apoptosis Induction in Human Thyroid Cancer Cell Line Thr.C1.PI33 by 3-Hydrogenkwadaphnin Isolated from Dendrostellera lessertii
Dendrostellera lessertii (Thymelaeaceae) is a toxic plant that grows in parts of Iran. The anti-proliferative properties of its crude methanol extract and one of its active components, 3-hydrogenkwadaphnin (3-HK), have been established using several cancer cell lines. Methods: In a further attempt to determine the mode of action, two groups of synchronously growing cells were treated with a sin...
متن کاملGnidilatimonoein from Daphne mucronata induced differentiation and apoptosis in leukemia cell lines
Among various treatments, plants play a crucial role in cancer chemotherapy. Base on literature data, different species of the thymeleaeceae family have been found to be good sources for anticancer agents. Therefore, our laboratory has initiated a research project on evaluating the anticancer properties of the Iranian medical plant, Daphne mucronata (Thymeleaeceae). Gnidilatimonoein is a new di...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian biomedical journalجلد ۱۳، شماره ۱، صفحات ۳۵-۴۲
کلمات کلیدی
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023